At present time, label-free biosensors are widely used in scientific and pharmaceutical research for analysis of biochemical reactions. This method allows to investigate on-rates and off-rates of different reactions between various substances such as RNA, DNA, proteins, including antibodies and antigens, viruses and bacteria. The applications of the novel biosensor chip based on graphene oxide will be demonstrated. Graphene oxide chips provide better sensitivity comparing to existing commercial sensor chips based on self-assembled monolayers and hydrogels.