In this talk I will present the results of the experiments on out-diffused silver nanoisland films. Using the field imprinting technique we were able to obtain separated single nanoislands, giving the opportunity to investigate their resonant properties, using dark-field spectroscopy. Simulations with DDA and COMSOL were compared with the experimental results. The field maps simulated at resonant wavelength for circular islands are shown. After these characterizations the applicability of the nanoisland film as an enhancement substrate for Raman spectroscopy was confirmed by measuring surface enhanced Raman spectroscopy (SERS) spectra of rhodamin 6G and bacteriorhodopsin.