10.00 – 10.45
Lecture 1. Investigation of muscle energetics using 31-phosphorus spectroscopy
The pathological process in neuromuscular disorders leads to a variety of alterations within muscle fibers including atrophy, inflammation, fatty infiltration etc. which can eventually affect muscle energy production. Taking advantage of development regarding MR scanners and dedicated radiofrequency antennas, 31-Phosphorus spectroscopy has emerged, a few decades ago, as a tool of choice for the non-invasive investigation of muscle energetics. Thanks to the detection of a few high-energy phosphate metabolites and the capacity of measuring intracellular pH, 31-P MRS has been used to document energy production in patients with muscle disorders and trained subjects. The purpose of the first lecture will be to present the 31-P MRS approach and the corresponding results regarding muscle energy production.
11.00 – 11.45
Lecture 2. MR imaging acquisition and post-processing issues
Over the last decade, dedicated MRI tools have been designed in order to quantify more or less specifically the histological abnormalities occurring in patients with neuromuscular disorders with the aim of providing sensitive biomarkers which could be used for diagnostic and therapeutic follow-up purposes. During the second lecture, the corresponding tools will be presented. Once the MR images are acquired a large amount of work is needed if one aims at tracking these abnormalities at the level of individual muscles or muscle groups. Atlas-based segmentation is a powerful method for automatic structural segmentation of several sub-structures in many organs. However, so far, very few studies have assessed this method for skeletal muscle segmentation. The second part of the lecture will be dedicated to the presentation of the multiple steps of an atlas-based pipeline we have developed for segmentation of quadriceps muscles from magnetic resonance images. Based on largely acknowledged parameters such as the DICE index, we will also illustrate the potential and the accuracy of different versions of the pipeline for longitudinal follow-up in healthy volunteers and in patients for whom an additional component of fat infiltration has to be taken into account.
Lecture 1. Investigation of muscle energetics using 31-phosphorus spectroscopy
The pathological process in neuromuscular disorders leads to a variety of alterations within muscle fibers including atrophy, inflammation, fatty infiltration etc. which can eventually affect muscle energy production. Taking advantage of development regarding MR scanners and dedicated radiofrequency antennas, 31-Phosphorus spectroscopy has emerged, a few decades ago, as a tool of choice for the non-invasive investigation of muscle energetics. Thanks to the detection of a few high-energy phosphate metabolites and the capacity of measuring intracellular pH, 31-P MRS has been used to document energy production in patients with muscle disorders and trained subjects. The purpose of the first lecture will be to present the 31-P MRS approach and the corresponding results regarding muscle energy production.
11.00 – 11.45
Lecture 2. MR imaging acquisition and post-processing issues
Over the last decade, dedicated MRI tools have been designed in order to quantify more or less specifically the histological abnormalities occurring in patients with neuromuscular disorders with the aim of providing sensitive biomarkers which could be used for diagnostic and therapeutic follow-up purposes. During the second lecture, the corresponding tools will be presented. Once the MR images are acquired a large amount of work is needed if one aims at tracking these abnormalities at the level of individual muscles or muscle groups. Atlas-based segmentation is a powerful method for automatic structural segmentation of several sub-structures in many organs. However, so far, very few studies have assessed this method for skeletal muscle segmentation. The second part of the lecture will be dedicated to the presentation of the multiple steps of an atlas-based pipeline we have developed for segmentation of quadriceps muscles from magnetic resonance images. Based on largely acknowledged parameters such as the DICE index, we will also illustrate the potential and the accuracy of different versions of the pipeline for longitudinal follow-up in healthy volunteers and in patients for whom an additional component of fat infiltration has to be taken into account.
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